Epub 2007 Feb 27. The plants were incubated in a growth chamber. doi: 10.1136/bmj.d4356.



The csaat1a, csaat1b, and double ddb1a csaat1a and b mutants had similar UV-B– and MMS-sensitive phenotypes, suggesting that CSAat1A and B function in the same pathway with DDB1A and that their functions in response to UV-B irradiation may not fully overlap. CMAJ. Catálogo de Plantas e Fungos do Brasil - versão 2010 (Volumes 1 e 2) Chase, M. W., and J. L. Reveal. Because the R2 peptides partially rescue their mutant phenotypes and contain a WDxR motif, it is possible that the WDxR motif also plays a role in the formation of a CSAat1A and B complex.

Single mutations at positions Asp-212, Trp-218, Asp-219, or Arg-221 in CSAat1A and B reduced their ability to interact with DDB1A (Figures 6B to 6E). Nome popular: Azulzinha; Tumbérgia-azul. Immunoprecipitation was performed using anti-Myc or anti-Flag agarose conjugate, and pull-down products were analyzed with anti-Flag or anti-Myc antibodies.

For DNA gel blot analysis, 10 mg of genomic DNA was digested with different combinations of restriction endonucleases, including BamHІ and SpeI (for At1g29230 probes); EcoRI and SalI (for At2g30360 probes); EcoRI and XhoI (for At3g23000 probes); PstI and XhoI (for At4g14580 probes); and BamHI and XbaI (for At5g10930 probes); and fractionated on an agarose gel (1% [w/v]). The WDxR motif is required and sufficient for the formation of the CSAat1A and B heterotetramer. The ddb1a homozygotes were identified using DDB1A gene-specific primers (DDB1A T-DNAF and DDB1A T-DNAR) and LBa1 and LBb1.

Trials. WB, immunoblot.

Fragments (R1, R2, and R3) were cloned into the pGADT7 vector; primers used for these constructs are listed in Supplemental Table 3 online. To determine whether the UV-B sensitive phenotype of the csaat1a-3 mutant is due to the decreased expression of CSAat1A, two Arabidopsis mutant lines (SALK_028416/csaat1a-1 and SALK_151258/csaat1a-2) with T-DNA insertions in the CSAat1A gene were obtained from the ABRC (Alonso et al., 2003). Bars = 1 cm.

Because CSAat1A and B share 92% amino acid sequence similarity, they may also be capable of forming homodimers in planta. USA.gov.

The Arabidopsis genome encodes two CSA-like genes that form heterotetramers, suggesting that a CUL4-DDB1ACSA E3 ligase is present in plants and plays a critical role in the response of the plant to UV-B irradiation.

Design:

The fresh weight of 10 seedlings in each group was measured. This work was supported by National Basic Research Program of China Grant 2006CB100100, National High Technology Research and Development Program of China 863 Grant 2003AA210100 to Y.G., and by U.S. Department of Energy/Energy Biosciences Grant DE-FG02-04ER15616 to K.S.S. To determine if CSAat1B also plays a role in DNA damage repair, two T-DNA insertion lines, SALK_152568/csaat1b-1 and SALK_144623/csaat1b-2, in CSAat1B were obtained from the ABRC, and the T-DNA insertion sites and the absence of full-length transcripts were determined (see Supplemental Figures 3A and 3B online). Get the latest research from NIH: https://www.nih.gov/coronavirus.

↵[W] Online version contains Web-only data. Seedlings were treated with UV-B light (UVP CL-1000M 34-0042-01, 302 nm, 130 μmol m−2 s−1) for 30 min and subsequently incubated for 5 d in a growth chamber. Natural history of warts. (A) and (B) Schematic representations of regions of the CSA1at1A and B proteins. CSA1at1A or B, CSAat1AD212A or BD212A, CSAat1AW218A or BW218A, CSAat1AD219A or BD219A, CSAat1AR221A or BR221A, CSAat1AL206A or BL206A, CSAat1AT208A, or BT208A and CSAat1AR216A or BR216A in pGADT7 were cotransformed with pGBKT7-DDB1A into yeast. The UVS90 protein contains four WD40 repeats and shares 54% sequence similarity with human CSA (see Supplemental Figure 2 online).

UV-B irradiation is a major genotoxic agent and has significant effects on plant growth and development. 2019 Jan-Mar;13(1):24-30. doi: 10.4103/JCAS.JCAS_62_19.

Taken together, these data indicate that the CUL4-DDB1CSACSB pathway exists in plants and functions in UV-induced DNA damage repair. CSA1at1A or CSA1at1B (B); D1, CSAat1AD212AW218A or BD212AW218A; D2, CSAat1AD212AD219A or BD212AD219A; D3, CSAat1AD212AR221A or BD212AR221A; D4, CSAat1AL206AD212A or BL206AD212A; T1, CSAat1AD212AW218AD219A or BD212AW218AD219A; T2, CSAat1AW218AD219AR221A or BW218AD219AR221A; and Q, CSAat1A D212AW218AD219AR221A or B D212AW218AD219AR221A in pGADT7 were cotransformed with pGBKT7-DDB1A into yeast. Primers used for to test for the presence of different fragments of CSAat1A and B (R1, R2, and R3) are listed in Supplemental Table 3 online. However, four CSAat1A and B proteins could provide a more efficient platform to recruit the UV damage repair machinery to the DNA lesion region and for degradation of CSB for post TCR recovery. Salicylic acid and the cryotherapy were equally effective for clearance of plantar warts. The plants were immediately transferred to continuous white light and harvested under green light after different repair times (0, 4, 8, 12, and 16 h). CSAat1A and B share significant sequence identity and have overlapping subcellular localization and expression patterns (see Supplemental Figures 2 and 4 online), suggesting that they functionally overlap. Columns in each panel represent serial decimal dilutions. Significant differences (P ≤ 0.05, Student’s t test) are indicated by different lowercase letters. All data represent means ± se of at least five replicate experiments (Student’s t test, **P < 0.01).